NAD-specific Glutamate Dehydrogenase of Neurospora crassa
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چکیده
منابع مشابه
Partial amino-acid sequence of NAD-specific glutamate dehydrogenase of Neurospora crassa.
Parts of the primary structure of the NAD-specific glutamate dehydrogenase [L-glutamate:NAD oxidoreductase (deaminating), EC 1.4.1.2] from Neurospora crassa are presented. Segments of the sequence representing 886 unique amino-acid residues have been determined; the largest contains 267 residues. There are only short regions of possible homology between this enzyme and the glutamate dehydrogena...
متن کاملSecondary structure predictions for the NAD-specific glutamate dehydrogenase of Neurospora crassa.
From the amino acid sequences of the three known fragments of the NAD-specific glutamate dehydrogenase of Neurospora crassa, the secondary structures have been predicted from the rules of Chou and Fasman (Chou, P.Y., and Fasman, G.D. (1979) Biophys. J. 26, 367-384). Comparison of these structures with those calculated for bovine glutamate dehydrogenase has shown that in the regions of homologou...
متن کاملNAD-specific glutamate dehydrogenase of Neurospora crassa. Limited action of trypsin and the presence of two distinct domains.
Limited tryptic hydrolysis of the NAD-specific glutamate dehydrogenase of Neurospora cmssa (EC 1.4.1.2) does not result in dissociation of the intact enzyme as shown by measurements of sedimentation velocity: szo,w = 14.1 S for the intact, and 13.6 S for the nicked enzyme. Sodium dodecyl sulfate and thiol treatment of the trypsin-treated enzyme revealed two major peptides: Peptides T-A (Mr = 47...
متن کاملAmino-acid sequence of NADP-specific glutamate dehydrogenase of neurospora crassa.
A tentative primary structure of the NADP-specific glutamate dehydrogenase [L-glutamate: NADP oxidoreductase (deaminating), EC 1.4.1.4] from Neurospora crassa has been determined. The proposed sequence contains 452 amino-acid residues in each of the identical subunits of the hexameric enzyme. Comparison of the sequence with that of the bovine liver enzyme reveals considerable homology in the am...
متن کاملNicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase of Neurospora.
Neurospora NADP-specific glutamate dehydrogenase that was treated with iodoacetate, iodoacetamide, or N-ethylmaleimide to block the thiol groups was cleaved with cyanogen bromide. Of the expected 10 peptides, based on a methionine content of 9 residues, 8 were obtained in pure form and 2 were handled as a mixture. The fragments ranged in size from 9 to 109 residues. In addition, there were isol...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85060-3